Who Framed Roger Rabbit? (1988)

Yosemite Sam is in two scenes of this cartoon-rich movie.  All of the cartoon characters are inserted into live-action scenes and given three-dimensional shading.

The first scene has Bob Hoskins seeing Sam flying down from the sky, with a burning butt.

Sam lands and tries to put himself out. He hops around and screams "My biscuits are burnin'!"

Sam plants his behind into a puddle of water, steam rushing up with red whiskers; he sighs relief.

At the end of the movie, Sam is seen among a crowd of other happy cartoon characters, when Toon Town is saved.

In celebration, Sam dances around happily, firing off his guns.

(Available on  Video and DVD.)